視頻來(lái)源：第二屆環(huán)狀RNA研究論壇精華視頻（circRNA）

人類環(huán)狀RNA鑒定及其在腫瘤中的潛在功能和意義 黃勝林-肽度TIMEDOO
黃勝林
博士，青年研究員，博士生導(dǎo)師
介紹來(lái)自：復(fù)旦大學(xué)生物醫(yī)學(xué)研究院
工作經(jīng)歷
青年研究員，復(fù)旦大學(xué)，生物醫(yī)學(xué)研究院（2013.9-今）
助理研究員，上海市腫瘤研究所, 癌基因及相關(guān)基因國(guó)家重點(diǎn)實(shí)驗(yàn)室（2009.7-2013.8）
博士后，美國(guó)斯坦福大學(xué)（2011.6-20013.7）
教育經(jīng)歷
博士，上海交通大學(xué)醫(yī)學(xué)院，腫瘤學(xué)（2006-2009）
碩士，上海交通大學(xué)醫(yī)學(xué)院，生物化學(xué)與分子生物學(xué)（2003-2006）
學(xué)士，福建醫(yī)科大學(xué)，臨床醫(yī)學(xué)（1998-2003）
所獲人才項(xiàng)目
浦江人才計(jì)劃，上海市科委，2014
上海市啟明星，上海市科委，2011
上海市衛(wèi)生計(jì)生系統(tǒng)優(yōu)秀青年人才，上海市衛(wèi)生系統(tǒng)，2011
研究方向
課題組運(yùn)用RNA高通量測(cè)序和生物信息分析，并結(jié)合各種實(shí)驗(yàn)手段，圍繞腫瘤轉(zhuǎn)錄組開(kāi)展了非編碼RNA與腫瘤特異轉(zhuǎn)錄本的鑒定及其病理生理效應(yīng)研究。解析了環(huán)狀RNA在腫瘤中的存在情況和表達(dá)特征，首次發(fā)現(xiàn)環(huán)狀RNA選擇性富集于外泌體，可作為潛在的腫瘤血液標(biāo)記物；發(fā)現(xiàn)腫瘤組織中存在著豐富的特異轉(zhuǎn)錄本，并具有重要的臨床意義和轉(zhuǎn)化價(jià)值。迄今發(fā)表SCI論文30余篇，他引率1600多次，主要研究成果發(fā)表于Nature Cell Biology, Cell Research, Nature Communications, Hepatology等專業(yè)雜志上。主持國(guó)家自然基金項(xiàng)目3項(xiàng)，申請(qǐng)發(fā)明專利1項(xiàng)，作為主要完成人獲教育部自然科學(xué)獎(jiǎng)二等獎(jiǎng)及上海醫(yī)學(xué)科技獎(jiǎng)二等獎(jiǎng)。
代表論文
  1. Dongbin Lyu, Shenglin Huang#. The emerging role and implication of human exonic circular RNA. RNA Biology. 2016. DOI: 10.1080/15476286.2016.1227904. (Invited Review)
  2. Qiupeng Zheng*, Chunyang Bao*, Weijie Guo, Shuyi Li, Jie Chen, Bin Chen, Yanting Luo, Dongbin Lyu, Yan Li, Guohai Shi, Linhui Liang, Jianren Gu, Xianghuo He#, Shenglin Huang#. Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs. Nature Communications. 2016;7:11215. DOI: 10.1038/ncomms11215.
  3. Chunyang Bao, Dongbin Lyu, Shenglin Huang#. Circular RNA expands its territory. Molecular & Cellular Oncology. 2015;3(2):e1084443.(Invited Commentary)
  4. Yan Li*, Qiupeng Zheng*, Chunyang Bao, Shuyi Li, Weijie Guo, Jiang Zhao, Di Chen, Jianren Gu, Xianghuo He#, Shenglin Huang#. Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis. Cell Research. 2015;25(8):981-4.
  5. Chunyang Bao*, Yan Li*, Lin Huan, Yuannv Zhang, Fangyu Zhao, Qifeng Wang, Linhui Liang, Jie Ding, Li Liu, Taoyang Chen, Jinjun Li, Ming Yao, Shenglin Huang#, Xianghuo He#. NF-kB signaling relieves negative regulation by miR-194 in hepatocellular carcinoma by suppressing the transcription factor HNF-1a. Science Signaling. 2015;8(387):ra75.
  6. Zheng Q, Cai X, Tan MH, Schaffert S, Arnold CP, Gong X, Chen CZ#, Huang S#. Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells. Biotechniques. 2014;57(3):115-24.
  7. Ding J*, Huang S*, Wang Y, Tian Q, Zha R, Shi H, Wang Q, Ge C, Chen T, Zhao Y, Liang L, Li J, He X. Genome-wide screening revealed that miR-195 targets the TNF-α/NF-κB pathway by downregulating IKKα and TAB3 in hepatocellular carcinoma. Hepatology, 2013; 58(2):654-66.
  8. Lin J, Huang S*, Wu S, Ding J, Zhao Y, Liang L, Tian Q, Zha R, Zhan R, He X. MicroRNA-423 promotes cell growth and regulates G(1)/S transition by targeting p21Cip1/Waf1 in hepatocellular carcinoma. Carcinogenesis. 2011; 32 (11): 1641-7.
  9. Huang S, He X. The role of microRNAs in liver cancer progression. Br J Cancer. 2011;104(2):235-40.
  10. Huang Z, Huang S, Wang Q, Liang L, Ni S, Wang L, Sheng W, He X, Du X. MicroRNA-95 Promotes Cell Proliferation and Targets Sorting Nexin 1 in Human Colorectal Carcinoma. Cancer Res. 2011; 71 (7): 2582-9.
  11. Huang S, Wu S, Ding J, Lin J, Wei L, Gu J, He X. ?MicroRNA-181a modulates gene expression of zinc finger family members by directly targeting their coding regions. Nucleic Acids Research. 2010; 38(20):7187-98.
  12. Ding J*, Huang S*, Wu S, Zhao Y, Liang L, Yan M, Ge C, Yao J, Chen T, Wan D, Wang H, Gu J, Yao M, Li J, Tu H, He X. Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA. Nature Cell Biology. 2010;12(4):390-9.
  13. Huang S, He X. MicroRNAs: Tiny RNA Molecules, Huge Driving Forces to Move the Cell. Protein & Cell. 2010, 1 (10): 916-926.
  14. Wu S*, Huang S*, Ding J, Zhao Y, Liang L, Liu T, Zhan R, He X. Multiple MicroRNAs Modulate p21Cip1/Waf1 Expression by Directly Targeting Its 3′ Untranslated Region. Oncogene. 2010;29(15):2302-8.
  15. Huang S, He X, Ding J, Liang L, Zhao Y, Zhang Z, Yao X, Pan Z, Zhang P, Li J, Wan D, Gu J. Upregulation of miR-23a~27a~24 Decreases Transforming Growth Factor-beta-induced Tumor Suppressive Activities in Human Hepatocellular Carcinoma Cells. International Journal of Cancer, 2008; 123 (4): 972-8.
  16. Shengli Li, Yuchen Li, Bing Chen, Jingjing Zhao, Shulin Yu, Yan Tang, Qiupeng Zheng, Yan Li, Peng Wang, Xianghuo He, Shenglin Huang. exoRBase: a database of circRNA, lncRNA and mRNA in human blood exosomes. Nucleic Acids Research. 2017 Oct 9.. gkx891,